|
KMID : 1149220070150020139
|
|
Korean Journal of Oriental Medical Prescription
2007 Volume.15 No. 2 p.139 ~ p.145
|
|
|
Antioxidant Effect of Cynomorii Herba on HepG2 Cells and Diphenyl-picryl-hydrazyl (DPPH) Radical Scavenging Activity
|
|
Chang Mun-Seog
Yang Woong-Mo
Kim Do-Rim
Park Eun-Hwa
Park Soo-Yeon
Park Seong-Kyu
|
|
|
Abstract
|
|
|
|
The purpose of this study was to investigate the anti-oxidant effect of Cynomorium songaricum. The extract of Cynomorii Herba was studied for diphenyl-picryl-hydrazyl (DPPH) radical scavenging activity, HepG2 cell viability and H2O2-induced cytotoxicity by a modified MTT assay. DPPH radical scavenging activity was measured after 30 minutes. The extract was tested by 1. 5, 10, 50, 100 and 500㎍/ml concentrations. HepG2 cell viability by a modified MTT assay was measured in the concentrations of 10, 50, 100, 250, 500 ug/ml for 24 h. The results showed that the extract scavenged DPPH radical up to 52.2% with 50 ug/ml concentration. The extract did not reduced the cell viability and H2O2-induced cytotoxicity (69.4%) was blocked by the extract in the concentrations of 50, 100, 250 and 500 ㎍/ml. In conclusion, the extract of Cynomorii Herba has potent antioxidant activity.
|
|
|
KEYWORD
|
|
|
Cynomorii herba, diphenyl-picryl-hydrazyl (DPPH), HepG2 cells, cytotoxicity, MTT assay
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|